Bioengineering, Functional and Molecular Imaging, Optoacoustics, Microscopy
What we investigate
Our laboratory is focused on development of new non-invasive functional and molecular imaging methods that can broadly impact pre-clinical research and clinical practice by delivering information presently not attainable with existing state-of-the-art imaging modalities.
Our research in more detail
Our work is motivated by the urge to study life in its unperturbed in vivo environment, which is essential for understanding the complex skin biology, disease state and progression. The multi-level biological interactions are difficult to predict or understand by making observations at a single spatial or temporal scale. Therefore, we are particularly interested in development of new imaging paradigms based on optoacoustics, functional ultrasonography, fluorescence microscopy, diffuse optics, optical coherence tomography, magnetic resonance imaging and their synergistic combinations to enable multi-scale observations with unprecedented spatio-temporal resolution and deep penetration into living intact organisms. Our group pioneered the multi-spectral optoacoustic tomography (MSOT) technique that uses pulses of light at different wavelengths, in a time-shared fashion, whereas the wavelengths are selected to sample a certain spectral characteristic in the absorption spectrum of intrinsic tissue chromophores (e.g. hemoglobin, melanin, lipids, water, collagen) or extrinsic reporter agent of interest. We have also demonstrated the clinical use of MSOT for label-free handheld dermatologic imaging applications, including real-time 3D functional mapping of pilosebaceous unit, non-melanoma skin cancers (Attia et al., 2017; Chuah et al., 2017), and basal cell carcinomas (Chuah et al., 2019).
SKINTEGRITY.CH Principal Investigators are underlined:
- Chuah SY, Ebrahim Attia AB, Ho CJH, Li X, Lee JSS, Tan MWP, Yong AA, Tan AWM, Razansky D, Olivo M, and Thng STJ (2019). Volumetric multispectral optoacoustic tomography for 3-dimensional reconstruction of skin tumors: a further evaluation with histopathologic correlation, J Invest Dermatol 39, 481-485.
- Deán-Ben XL, Gottschalk S, McLarney B, Shoham S and Razansky D (2017). Advanced optoacoustic methods for multi-scale imaging of in vivo dynamics. Chem Soc Rev, 46, 2158—2198.
- Ford SJ, Bigliardi PL, Sardella T, Urich A, Burton NC, Kacprowicz M, Olivo M and Razansky D. (2016) Structural and functional analysis of intact hair follicles and pilosebaceous units by volumetric multispectral optoacoustic tomography. J Invest Dermatol, 136, 753-761.
- Razansky D, Distel M, Vinegoni C, Ma R, Perrimon N, Köster RW and Ntziachristos V (2009). Multi-spectral optoacoustic tomography of deep-seated fluorescent proteins in-vivo. Nature Phot, 3, 412-417.